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Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

Virulence gene- and pandemic group-specific marker profiling of clinical Vibrio parahaemolyticus isolates

Carolyn E Meador1, Michele M Parsons, Cheryl A Bopp

  • 1Naval Research Laboratory, Center for Bio/Molecular Science & Engineering, 4555 Overlook Avenue SW, Bldg. 30, Code 6910, Washington, DC 20375, USA. gvora@cbmse.nrl.navy.mil

Journal of Clinical Microbiology
|February 16, 2007
PubMed

Insights

Pandemic Vibrio parahaemolyticus strains are not solely defined by the chromosome 2 type III secretion system. This genetic marker is also present in non-pandemic, Kanagawa-negative clinical isolates.

Area of Science:

  • Microbiology
  • Genetics
  • Infectious Diseases

Background:

  • Vibrio parahaemolyticus causes foodborne illness and has emerged in pandemic strains like O3:K6.
  • Understanding genetic markers differentiates pandemic from non-pandemic strains.

Purpose of the Study:

  • To investigate virulence factors and genetic markers in Vibrio parahaemolyticus.
  • To determine if the chromosome 2 type III secretion system is specific to pandemic O3:K6 strains.

Main Methods:

  • Microarray analysis of 48 human clinical Vibrio parahaemolyticus isolates (1997-2005).
  • Detection of known virulence factors and genetic markers.

Main Results:

  • The chromosome 2 type III secretion system was found in both pandemic and non-pandemic strains.
  • This system was present in tdh-negative (Kanagawa-negative) clinical isolates, not exclusively in pandemic strains.

Conclusions:

  • The chromosome 2 type III secretion system is not a specific marker for pandemic Vibrio parahaemolyticus O3:K6.
  • Results indicate significant genetic diversity within Vibrio parahaemolyticus, refining the definition of pandemic strains.

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