Related Experiment Video
Updated: Jul 16, 2026

Application of CRISPR Interference (CRISPRi) for Gene Silencing in Pathogenic Species of Leptospira
Published on: August 14, 2021
Optimization of culture of Leptospira from humans with leptospirosis
Vanaporn Wuthiekanun1, Wirongrong Chierakul, Direk Limmathurotsakul
1Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Road, Bangkok 10400, Thailand. lek@tropmedres.ac
Abstract:
A prospective study of 989 patients with acute febrile illness was performed in northeast Thailand to define the yield of Leptospira from four different types of blood sample. Based on a comparison of the yields from whole blood, surface plasma, deposit from spun plasma, and clotted blood samples from 80 patients with culture-proven leptospirosis, we suggest a sampling strategy in which culture is performed using whole blood and deposit from spun plasma.

