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Related Experiment Videos

[How to develop custom-designed fluorescence probes for molecular imaging].

Tasuku Ueno1, Yasuteru Urano, Tetsuo Nagano

  • 1Graduate School of Pharmaceutical Sciences, The University of Tokyo.

Nihon Rinsho. Japanese Journal of Clinical Medicine
|February 17, 2007
PubMed
Summary
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Researchers developed novel fluorescence probes for real-time cellular imaging by controlling photoinduced electron transfer (PeT) in fluorescein derivatives. This breakthrough enables visualization of diverse biomolecules, advancing live-cell dynamic process studies.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Chemical Biology

Context:

  • Fluorescence imaging is crucial for observing dynamic intracellular processes in living cells.
  • Current limitations exist in visualizing a wide range of biomolecules due to a lack of flexible fluorescence probe design strategies.
  • Fluorescein, a common fluorescence probe core, can be viewed as an electron donor/fluorophore acceptor system.

Purpose:

  • To develop a flexible design strategy for fluorescence probes based on the photoinduced electron transfer (PeT) mechanism.
  • To modulate and predict the fluorescence properties of fluorescein derivatives by controlling PeT rates.
  • To create novel probes for imaging various intracellular targets.

Summary:

  • Elucidated fluorescein as a directly linked electron donor/fluorophore acceptor system.

Related Experiment Videos

  • Demonstrated control over fluorescence properties by modulating photoinduced electron transfer (PeT) rates.
  • Developed over twenty probes for imaging nitric oxide, beta-galactosidase, reactive oxygen species, and zinc ions using rational PeT-based design.

    • Impact:

    • Enabled precise and rational design of fluorescence probes based on the PeT mechanism.
    • Expanded the range of biomolecules that can be visualized in live-cell imaging.
    • Facilitated continuous observation of dynamic intracellular processes with enhanced molecular targeting.