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Toxoplasma gondii: the role of a 30-kDa surface protein in host cell invasion
1Department of Pure and Applied Biology, University of Leeds, U.K.
Experimental Parasitology
|February 1, 1992
Abstract:
C1E3, a monoclonal antibody recognizing protein P30, a major surface antigen of Toxoplasma gondii tachyzoites, was shown to have a consistent effect on invasion in adult bovine kidney cells. In 10 replicate assays, the overall invasion was reduced to 37% of control values (P less than 0.0001). These results support the role of a functional role for P30 in mediating invasion.
Insights
Monoclonal antibody C1E3 significantly reduced Toxoplasma gondii invasion by targeting the P30 surface antigen. This confirms P30
Area of Science:
- Parasitology
- Immunology
- Cell Biology
Background:
- Toxoplasma gondii is a significant protozoan parasite.
- The P30 protein is a major surface antigen on tachyzoites.
- Understanding invasion mechanisms is crucial for therapeutic development.
Purpose of the Study:
- To investigate the role of the P30 antigen in T. gondii invasion.
- To evaluate the effect of the C1E3 monoclonal antibody on parasite invasion.
Main Methods:
- Utilized the C1E3 monoclonal antibody, which targets the P30 protein.
- Performed invasion assays using adult bovine kidney cells.
- Conducted 10 replicate experiments to assess invasion rates.
Main Results:
- The C1E3 antibody consistently reduced T. gondii tachyzoite invasion.
- Invasion was decreased to 37% of control levels (P < 0.0001).
- Demonstrated a statistically significant inhibitory effect on parasite entry.
Conclusions:
- The P30 surface antigen plays a functional role in T. gondii invasion.
- Monoclonal antibody C1E3 effectively inhibits parasite invasion, validating P30's role.