Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Radical Autoxidation01:20

Radical Autoxidation

The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Bioactivation and Tissue Toxicity01:25

Bioactivation and Tissue Toxicity

Bioactivation is a metabolic process that transforms less reactive substances into highly reactive metabolites, initiating tissue toxicity. This transformation can lead to various toxic effects, including carcinogenesis and teratogenesis. Reactive metabolites are classified into two main types: electrophiles and free radicals.Electrophiles are electron-deficient species and are produced primarily by the enzyme cytochrome P-450 during the metabolism of compounds containing carbon, nitrogen, or...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The diagnostic accuracy of interferon-gamma release assay for TB infection in children under 5 years: a systematic review with meta-analysis.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2026
Same author

From microstructure to mechanics: A multi-constituent micro-scale computational model of arterial tissue.

Computers in biology and medicine·2025
Same author

Developmental venous anomalies: benign in nature, potentially debilitating for life.

Acta neurologica Belgica·2025
Same author

Mycothione reductase as a potential target in the fight against <i>Mycobacterium abscessus</i> infections.

mSphere·2023
Same author

Development and validation of an HPLC-DAD method for Rapid quantification of vasicine in <i>Adhatoda vasica</i> leaves and commercial products.

Heliyon·2022
Same author

The search for novel treatment strategies for Streptococcus pneumoniae infections.

FEMS microbiology reviews·2021

Related Experiment Video

Updated: Jul 16, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

Challenges and pitfalls in antioxidant research.

N Hermans1, P Cos, L Maes

  • 1Laboratory of Nutrition and Functional Food Science, Department of Pharmaceutical Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. nina.hermans@ua.ac.be

Current Medicinal Chemistry
|February 20, 2007
PubMed
Summary

This review highlights pitfalls in antioxidant research, emphasizing the need for multiple validated assays for accurate in vitro and in vivo assessments. Proper methodology ensures reliable evaluation of antioxidant compounds and their health benefits.

More Related Videos

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

Valorization of the Red Seaweed Gracilaria gracilis Through a Biorefinery Approach
10:18

Valorization of the Red Seaweed Gracilaria gracilis Through a Biorefinery Approach

Published on: November 21, 2023

Related Experiment Videos

Last Updated: Jul 16, 2026

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
09:33

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors

Published on: February 7, 2018

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

Valorization of the Red Seaweed Gracilaria gracilis Through a Biorefinery Approach
10:18

Valorization of the Red Seaweed Gracilaria gracilis Through a Biorefinery Approach

Published on: November 21, 2023

Area of Science:

  • Biochemistry
  • Pharmacology
  • Nutritional Science

Background:

  • Extensive research has explored the health benefits of antioxidants.
  • Numerous synthetic and natural compounds have been assessed for antioxidant properties.
  • Current research often employs limited, poorly validated, and non-specific assays.

Purpose of the Study:

  • To review common pitfalls in in vitro and in vivo antioxidant research.
  • To discuss essential methodologies for accurate antioxidant evaluation.
  • To underscore the importance of robust assay selection and validation.

Main Methods:

  • Discussion of various in vitro antioxidant assays, including radical scavenging and fingerprinting techniques.
  • Exploration of in vivo research methodologies for assessing antioxidant activity.
  • Analysis of limitations and challenges in current antioxidant assay validation.

Main Results:

  • A diverse panel of in vitro assays is indispensable for reliable antioxidant characterization.
  • In vivo studies are crucial for understanding absorption, distribution, metabolism, and excretion (ADME) of antioxidants.
  • Representative animal models and validated assays are vital for in vivo antioxidant research.

Conclusions:

  • Accurate antioxidant research requires a comprehensive approach using multiple validated assays.
  • In vitro findings must be complemented by in vivo studies to confirm efficacy and bioavailability.
  • Addressing methodological pitfalls is critical for advancing the understanding of antioxidant health benefits.