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Natural Product Discovery with LC-MS/MS Diagnostic Fragmentation Filtering: Application for Microcystin Analysis
Published on: May 31, 2019
Microbial degradation of cyanobacterial cyclic peptides
Hajime Kato1, Susumu Y Imanishi, Kiyomi Tsuji
1Graduate School of Environmental and Human Sciences, Meijo University, 150, Yagotoyama, Tempaku-ku, Nagoya 468-8503, Japan. q0691501@ccmailg.meijo-u.ac.jp
Abstract:
Bacterial strain B-9 possesses hydrolytic enzymes capable of degrading microcystins (MCs) and nodularin that are toxic cyclic peptides produced by cyanobacteria. In the present study, the degradation activities of the cell extract of B-9 against non-toxic cyanobacterial cyclic peptides other than the MCs and nodularin were investigated, and the degradation products were analyzed by liquid chromatography/ion trap tandem mass spectrometry (LC/ITMS). It was confirmed that B-9 could also degrade these cyanobacterial cyclic peptides by hydrolysis of their peptide bonds. These results indicated that this bacterium possesses a very unique hydrolytic activity that can degrade structurally different cyclic peptides and that this may be effective for the detoxification of hazardous cyclic peptides.
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