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Updated: Jul 16, 2026
11:48
Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
Global analysis of alternative splicing during T-cell activation
Joanna Y Ip1, Alan Tong, Qun Pan
1Banting and Best Department of Medical Research, University of Toronto, Toronto, ONT, Canada.
RNA (New York, N.Y.)
|February 20, 2007
Summary
Alternative splicing (AS) significantly impacts immune responses. This study reveals numerous novel AS events during T-cell activation, suggesting a broad regulatory role for AS in the mammalian immune system.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- The role of alternative splicing (AS) in immune responses is not well understood.
- T-cell activation is a critical process in the mammalian immune system.
Purpose of the Study:
- To investigate changes in alternative splicing during T-cell activation.
- To identify novel alternative splicing events involved in immune responses.
Main Methods:
- Quantitative alternative splicing microarray profiling was employed.
- Jurkat cells and primary human lymphocytes (CD4+ and CD8+) were used to study T-cell activation.
Main Results:
- Approximately 10-15% of profiled alternative exons showed significant changes (>10%) in inclusion levels upon activation.
- Genes with differential AS levels were distinct from those with differential transcript levels, yet shared overlapping functional roles.
- Novel AS events were identified in key T-cell activation genes, and these changes were conserved in primary human lymphocytes.
Conclusions:
- Alternative splicing plays an extensive and previously underappreciated role in regulating mammalian immune responses.
- AS is a critical layer of gene regulation during T-cell activation, impacting diverse cellular functions.
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