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Related Experiment Videos

Quantitative imaging of lymph function.

Ruchi Sharma1, Wei Wang, John C Rasmussen

  • 1Division of Molecular Imaging, Department of Radiology, Baylor College of Medicine, Houston, TX 77030, USA.

American Journal of Physiology. Heart and Circulatory Physiology
|February 20, 2007
PubMed
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Near-infrared fluorescence imaging successfully visualized lymphatic function in swine using indocyanine green (ICG) and a hyaluronan conjugate. This technique shows potential for diagnosing lymph-related disorders.

Area of Science:

  • Biomedical Imaging
  • Lymphatic System Research
  • Optical Diagnostics

Background:

  • Lymphatic imaging is crucial for diagnosing disorders.
  • Current methods have limitations in visualizing lymphatic function.
  • Near-infrared (NIR) fluorescence offers potential for improved lymphatic visualization.

Purpose of the Study:

  • To demonstrate functional lymphatic imaging in swine using NIR fluorescence.
  • To evaluate indocyanine green (ICG) and a hyaluronan-ICG conjugate for lymph imaging.
  • To assess the agents' ability to visualize lymph trafficking and propulsion.

Main Methods:

  • Intradermal administration of ICG and a hyaluronan-ICG conjugate in anesthetized swine.
  • NIR fluorescence imaging with 780 nm excitation and 830 nm emission detection.

Related Experiment Videos

  • Analysis of dye trafficking, lymph vessel visualization, and propulsion dynamics.

    • Main Results:

    • Successfully imaged ICG trafficking from injection site to lymph nodes up to 3 cm deep.
    • Observed "packets" of lymph propulsion in vessels at specific velocities and frequencies.
    • Hyaluronan conjugate showed immediate dye progression without distinct propulsion.
    • Imaging required microgram quantities of the NIR agents.

    Conclusions:

    • NIR fluorescence imaging with ICG and conjugates can visualize lymphatic function.
    • The technique demonstrates lymph trafficking and propulsion dynamics.
    • Microgram quantities of NIR agents show promise for clinical imaging of lymph disorders.