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Related Experiment Videos

Spectral-domain optical coherence phase and multiphoton microscopy.

Chulmin Joo1, Ki Hean Kim, Johannes F de Boer

  • 1Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA. cmjoo@mit.edu

Optics Letters
|February 20, 2007
PubMed
Summary
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This study introduces a novel combined imaging system for simultaneous quantitative phase contrast and multiphoton fluorescence microscopy. The technique provides high-resolution structural and functional imaging of transparent specimens, visualizing cellular details like actin filaments.

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Cell Biology

Background:

  • Quantitative phase contrast microscopy offers label-free imaging of transparent biological samples.
  • Multiphoton fluorescence microscopy provides deep tissue penetration and specific molecular labeling.
  • Simultaneous imaging modalities can enhance structural and functional insights into biological systems.

Purpose of the Study:

  • To develop and demonstrate a combined spectral-domain optical coherence phase microscopy and multiphoton microscopy system.
  • To achieve simultaneous quantitative phase contrast and multiphoton fluorescence imaging.
  • To validate the system's capability for high-resolution imaging of cellular structures.

Main Methods:

  • Integration of spectral-domain optical coherence phase microscopy and multiphoton microscopy.

Related Experiment Videos

  • Utilizing two distinct light sources for efficient operation of both modalities.
  • Imaging transparent specimens in the epidirection.
  • Demonstration of subnanometer phase stability in quantitative phase contrast imaging.

    • Main Results:

    • Successful simultaneous acquisition of quantitative phase contrast and multiphoton fluorescence images.
    • Generation of high-resolution structural and functional images of transparent specimens.
    • Visualization of actin filaments in a fixed cell specimen using phase contrast.
    • Confirmation of actin filament visualization through concurrent multiphoton fluorescence imaging.

    Conclusions:

    • The combined imaging system enables simultaneous structural and functional analysis of biological specimens.
    • The instrument achieves high phase stability for quantitative phase contrast imaging.
    • This multimodal approach offers a powerful tool for detailed cellular imaging and research.