Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surface plasmon resonance imaging using a high numerical aperture microscope objective.

Bo Huang1, Fang Yu, Richard N Zare

  • 1Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.

Analytical Chemistry
|February 21, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of crt and mdr-1 mutations in Plasmodium falciparum isolates from Grande Comore island after withdrawal of chloroquine.

Malaria journal·2016
Same author

Treatment of proximal and middle one-third humeral fractures with lateral distal tibial helical plate.

European journal of orthopaedic surgery & traumatology : orthopedie traumatologie·2016
Same author

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports·2016
Same author

Tumor Exosomal RNAs Promote Lung Pre-metastatic Niche Formation by Activating Alveolar Epithelial TLR3 to Recruit Neutrophils.

Cancer cell·2016
Same author

Ultra-compact strain- and temperature-insensitive torsion sensor based on a line-by-line inscribed phase-shifted FBG.

Optics express·2016
Same author

The Safety and Immunological Effects of rAd5-EBV-LMP2 Vaccine in Nasopharyngeal Carcinoma Patients: A Phase I Clinical Trial and Two-Year Follow-Up.

Chemical & pharmaceutical bulletin·2016
Same journal

Strain-Level Food Surveillance of <i>Escherichia coli</i> Using a Specific-Nonspecific Hybrid Sensor Array Strategy.

Analytical chemistry·2026
Same journal

A Field-Portable Fe(IV)-Mediated Competitive Quenching Chemiluminescence Platform with a Synchronous Y-Shaped Flow-through Cell for Broad-Spectrum Quantification of Volatile Phenols.

Analytical chemistry·2026
Same journal

Single-Molecule Characterization of CRISPR-Cas12a for Amplification-Free Genetic Testing.

Analytical chemistry·2026
Same journal

Integrated Acoustofluidic Manipulation and Oscillation-Stabilized Magnetic Relaxation Biosensing for <i>Salmonella</i> Detection.

Analytical chemistry·2026
Same journal

A Self-Powered Sensing Platform Based on the Janus Heterostructure for Machine Learning-Assisted Dual-Mode Detection of 17β-Estradiol.

Analytical chemistry·2026
Same journal

Large Language Model-Generated Dietary Metabolite Biomarker Database Drives Deep Annotation of the Human Diet Metabolome.

Analytical chemistry·2026
See all related articles

We developed a novel surface plasmon resonance (SPR) microscope achieving high-resolution imaging. This advanced optical microscopy technique offers detailed insights into surface properties with improved spatial accuracy.

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Nanotechnology

Background:

  • Surface plasmon resonance (SPR) is a label-free optical technique used for detecting surface binding events.
  • Conventional SPR microscopy often faces limitations in spatial resolution and image distortion.

Purpose of the Study:

  • To design and construct a high-resolution SPR microscope.
  • To achieve diffraction-limited spatial resolution in SPR imaging.
  • To enable distortion-free, angle-resolved SPR imaging for detailed surface analysis.

Main Methods:

  • Utilized a high numerical aperture objective from a commercial inverted optical microscope.
  • Implemented methods to shorten surface plasmon propagation length.
  • Developed objective-type SPR imaging for angle-resolved analysis.

Related Experiment Videos

Main Results:

  • Achieved diffraction-limited spatial resolution in the transverse direction.
  • Obtained near-diffraction-limited resolution in the longitudinal direction.
  • Demonstrated distortion-free angle-resolved SPR imaging on a pixel-by-pixel basis.

Conclusions:

  • The developed SPR microscope offers superior spatial resolution and imaging quality.
  • This technique provides high-resolution, angle-dependent information about surface properties.
  • The objective-type configuration enhances the utility of SPR microscopy for various applications.