Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nucleosome Remodeling02:54

Nucleosome Remodeling

8.7K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
8.7K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

9.5K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pervasive and programmed nucleosome distortion on single chromatin fibres.

Nature·2026
Same author

Preferential formation of NUP98-KDM5A condensates at specific H3K4me3-rich loci drives leukemogenic gene expression.

bioRxiv : the preprint server for biology·2026
Same author

HP1γ self-assembles and cooperates with KAP1 in repression of long noncoding RNA AI662270 in ESCs.

Cell reports·2026
Same author

ATP-dependent remodeling of chromatin condensates reveals distinct mesoscale outcomes.

Science (New York, N.Y.)·2025
Same author

HMGB1 deforms nucleosomal DNA to generate a dynamic chromatin environment counteracting the effects of linker histone.

Science advances·2025
Same author

Two stages of substrate discrimination dictate selectivity in the Escherichia coli MetNI-Q ABC transporter system.

The Journal of biological chemistry·2025
Same journal

An accessible, absorbance-based plate reader assay to assess cumulative exposure of blood plasma & serum to thawed conditions.

Methods (San Diego, Calif.)·2026
Same journal

EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples.

Methods (San Diego, Calif.)·2026
Same journal

Single-Molecule methods to investigate mechanisms of transcription by RNA polymerase of Mycobacterium tuberculosis.

Methods (San Diego, Calif.)·2026
Same journal

Detection and sequencing of Usutu virus during mosquito surveillance: Use of multiple assays and techniques for identification at low levels.

Methods (San Diego, Calif.)·2026
Same journal

Experimental validation of an AI-driven digital healthcare platform for oral health behavior and plaque assessment among vietnamese children.

Methods (San Diego, Calif.)·2026
Same journal

Zeta potential: An efficient and cost-effective alternative for investigating cell-surface interactions.

Methods (San Diego, Calif.)·2026
See all related articles

Related Experiment Video

Updated: Apr 30, 2026

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
10:14

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Published on: October 25, 2014

14.0K

FRET-based methods to study ATP-dependent changes in chromatin structure.

Janet G Yang1, Geeta J Narlikar

  • 1Department of Biochemistry and Biophysics, University of California, San Francisco, 600 16th Street, San Francisco, CA 94158, USA.

Methods (San Diego, Calif.)
|February 21, 2007
PubMed
Summary
This summary is machine-generated.

Researchers developed a new FRET-based method to observe DNA remodeling on a single histone octamer in real-time. This technique offers a non-perturbing way to study chromatin remodeling mechanisms and enzyme activity.

More Related Videos

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

7.8K
Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
09:26

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

4.5K

Related Experiment Videos

Last Updated: Apr 30, 2026

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes
10:14

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

Published on: October 25, 2014

14.0K
Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
07:41

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes

Published on: October 2, 2017

7.8K
Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
09:26

Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis

Published on: March 23, 2021

4.5K

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Biochemistry

Background:

  • DNA packaging into chromatin regulates essential nuclear processes like replication, repair, and transcription.
  • ATP-dependent chromatin-remodeling enzymes are key regulators, modulating nucleosomal DNA accessibility.
  • Chromatin remodeling influences large-scale structures (heterochromatin) and specific DNA region exposure.

Purpose of the Study:

  • To elucidate the mechanisms of chromatin remodeling.
  • To introduce a novel method for real-time observation of single histone octamer remodeling on DNA.

Main Methods:

  • Development of a Förster Resonance Energy Transfer (FRET)-based technique.
  • Quantitative tracking of DNA movement relative to the histone octamer in solution.
  • Real-time monitoring of conformational changes within the nucleosome.

Main Results:

  • Demonstrated a non-perturbing, solution-based approach for studying chromatin remodeling.
  • Enabled quantitative, real-time tracking of DNA-histone octamer dynamics.
  • Validated the method's applicability to various chromatin remodeling complexes.

Conclusions:

  • The FRET-based method provides a powerful tool for understanding chromatin remodeling mechanisms.
  • This technique facilitates the study of nucleosome dynamics and the enzymatic activity of remodeling complexes.
  • The approach is adaptable for investigating diverse conformational changes within the nucleosome.