Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

Nucleosome Remodeling

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...

Studying the Cytoskeleton

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...

Spreading of Chromatin Modifications

Spreading of Chromatin Modifications

The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Design of a novel antisymmetric coil array for parallel transmit cardiac MRI in pigs at 7 T.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2019

Same author

Measles virus spread initiated at international mass gatherings in Europe, 2011.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2014

Same author

Rise in the number of notified human hantavirus infections since October 2011 in Baden-Wurttemberg, Germany.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2012

Same author

Spotlight on measles 2010: Measles outbreak among travellers returning from a mass gathering, Germany, September to October 2010.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2010

Same author

Intrinsic promoter nucleosome stability/dynamics variations support a novel targeting mechanism.

Biochemistry·2009

Same author

DNA sequence-dependent variation in nucleosome structure, stability, and dynamics detected by a FRET-based analysis.

Biochemistry and cell biology = Biochimie et biologie cellulaire·2009

Search research articles

Related Experiment Video

Updated: Jul 16, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

Using atomic force microscopy to study chromatin structure and nucleosome remodeling.

D Lohr¹, R Bash, H Wang

¹Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-1604, USA. dlohr@asu.edu

Methods (San Diego, Calif.)

|February 21, 2007

Summary

Atomic force microscopy (AFM) allows direct imaging of single molecules in solution. This study adapted AFM techniques to analyze nucleosome arrays, revealing insights into their structure and remodeling by the Swi-Snf complex.

More Related Videos

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Published on: September 6, 2024

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Related Experiment Videos

Last Updated: Jul 16, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

In Situ Nucleosome Assembly for Single-Molecule Correlative Force and Fluorescence Microscopy

Published on: September 6, 2024

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

Area of Science:

Biophysics
Molecular Biology
Microscopy

Background:

Atomic force microscopy (AFM) offers direct visualization of single molecules in solution.
Understanding nucleosome array properties is crucial for comprehending DNA packaging and regulation.

Purpose of the Study:

To adapt AFM methods for detailed analysis of nucleosome arrays.
To investigate nucleosome features like DNA-histone binding, stability, and the impact of acetylation.
To track nucleosome remodeling by the human Swi-Snf complex.

Main Methods:

Utilized specialized AFM techniques for repetitive imaging in liquid.
Adapted environmental control for samples during imaging.
Developed methods for identifying specific molecules in complex samples.

Main Results:

Successfully analyzed DNA-histone binding strength and nucleosome stability in arrays.
Compared nucleosome features across different array types.
Monitored the dynamic response of nucleosomes to Swi-Snf remodeling.

Conclusions:

Advanced AFM methodologies enable in-depth studies of nucleosome array dynamics.
These techniques provide unique insights into chromatin structure and remodeling processes.
The study highlights AFM's power in investigating biological material properties and functions.