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Updated: Jul 16, 2026
09:41
Quantification of Monocyte Transmigration and Foam Cell Formation from Individuals with Chronic Inflammatory Conditions
Published on: October 17, 2017
Effect of FXIII on monocyte and fibroblast function
Rima Dardik1, Tanya Krapp, Esther Rosenthal
1Institute of Thrombosis and Hemostasis, Sheba Medical Center, Tel Hashomer.
Summary
Factor XIII (FXIIIa) promotes tissue repair by enhancing monocyte and fibroblast activity. This protein stimulates cell migration and proliferation while inhibiting apoptosis, supporting its crucial role in healing.
Area of Science:
- Biochemistry
- Cell Biology
- Angiogenesis Research
Background:
- Factor XIII (FXIII) is a plasma transglutaminase crucial for blood coagulation.
- Thrombin-activated FXIII (FXIIIa) crosslinks fibrin, maturing blood clots.
- Previous studies demonstrated FXIIIa's pro-angiogenic effects on endothelial cells.
Purpose of the Study:
- To investigate the effects of FXIIIa on monocytes and fibroblasts, key cells in tissue repair.
- To elucidate the molecular mechanisms underlying FXIIIa's influence on these cells.
Main Methods:
- Assessing monocyte and fibroblast migration and proliferation.
- Evaluating apoptosis rates in monocytes and fibroblasts.
- Investigating FXIIIa binding to alpha(v)beta(3) integrin.
- Analyzing cJun and TSP-1 expression levels.
Main Results:
- FXIIIa significantly enhanced monocyte and fibroblast migration and proliferation.
- FXIIIa inhibited apoptosis in both cell types.
- FXIIIa's effects were mediated by alpha(v)beta(3) integrin binding, leading to cJun upregulation and TSP-1 downregulation.
Conclusions:
- FXIIIa promotes critical cellular activities involved in tissue repair.
- The findings support a significant role for Factor XIII in tissue repair processes beyond hemostasis.
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