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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

Pre-mRNA Processing: RNA Splicing

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Rous Sarcoma Virus (RSV) and Cancer

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Related Experiment Video

Updated: Jul 16, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

The gene encoding the splicing factor SF2/ASF is a proto-oncogene.

Rotem Karni¹, Elisa de Stanchina, Scott W Lowe

¹Cold Spring Harbor Laboratory, PO Box 100, Cold Spring Harbor, New York 11724, USA.

Nature Structural & Molecular Biology

|February 21, 2007

Summary

The splicing factor SF2/ASF, when overexpressed, acts as an oncoprotein driving cancer development by altering tumor suppressor and kinase splicing. Targeting SF2/ASF offers a potential cancer therapy strategy.

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Last Updated: Jul 16, 2026

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

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Area of Science:

Molecular Biology
Oncology
Gene Regulation

Background:

Alternative splicing significantly impacts the expression of oncogenes and tumor suppressors.
The role of alternative splicing factors in cancer development remains an area of active investigation.

Purpose of the Study:

To investigate the involvement of splicing factors in cancer.
To determine if SF2/ASF functions as an oncoprotein and explore its therapeutic potential.

Main Methods:

Analysis of SF2/ASF expression in human tumors.
Cell transformation assays using rodent fibroblasts.
Investigation of SF2/ASF's control over alternative splicing of BIN1, MNK2, and S6K1.
In vitro and in vivo knockdown experiments.

Main Results:

SF2/ASF (Splicing Factor 2/Alternative Splicing) is upregulated in human tumors, partly due to SFRS1 gene amplification.
SF2/ASF overexpression transforms rodent fibroblasts into sarcoma-forming cells.
SF2/ASF regulates splicing of BIN1, MNK2, and S6K1, generating isoforms with altered functions.
Knockdown of SF2/ASF or S6K1 isoform 2 reverses oncogenic transformation.

Conclusions:

SF2/ASF functions as an oncoprotein by manipulating alternative splicing pathways.
SF2/ASF-driven oncogenesis involves specific isoforms of BIN1, MNK2, and S6K1.
SF2/ASF represents a promising therapeutic target for various cancers.