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Updated: Jul 16, 2026

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
Published on: June 11, 2010
Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted
Su-Sung Kim1, Dong-Il Shin, Hee-Sung Park
1Department of Biotechnology, Catholic University of Daegu, Kyungsan, Kyungbuk, Korea.
Abstract:
A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.

