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Leftward ribosome frameshifting at a hungry codon.

J A Gallant1, D Lindsley

  • 1Department of Genetics, University of Washington, Seattle 98195.

Journal of Molecular Biology
|January 5, 1992
PubMed
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Aminoacyl-tRNA limitation can cause frameshift suppression by inducing a ribosome frameshift. This occurs at a hungry AAG codon, shifting translation one base to the left.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Frameshift mutations alter gene reading frames, impacting protein synthesis.
  • Aminoacyl-tRNA availability is crucial for accurate translation.
  • Previous studies linked tRNA limitation to frameshift suppression.

Purpose of the Study:

  • To elucidate the mechanism of phenotypic suppression caused by aminoacyl-tRNA limitation.
  • To investigate ribosome frameshifting at specific codons.
  • To determine the directionality of frameshifting.

Main Methods:

  • Inducing aminoacyl-tRNA limitation.
  • Analyzing protein products for sequence alterations.
  • Modifying gene sequences to study frameshift events.

Related Experiment Videos

Main Results:

  • Demonstrated phenotypic suppression via ribosome frameshifting.
  • Identified a hungry AAG codon (lysyl-tRNA deficiency) as a frameshift trigger.
  • Confirmed a one-base leftward frameshift at the hungry codon.

Conclusions:

  • Ribosome frameshifting at deficient tRNA codons is a mechanism for frameshift suppression.
  • This frameshift event allows translation to continue in an altered reading frame.
  • Understanding this process aids in studying gene expression and mutation.