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Ceramic Omnidirectional Bioprinting in Cell-Laden Suspensions for the Generation of Bone Analogs
Published on: August 8, 2022
In vitro osteogenesis on a highly bioactive glass-ceramic (Biosilicate)
João Moura1, Lucas Novaes Teixeira, Christian Ravagnani
1Cell Culture Laboratory, Faculty of Dentistry of Ribeirão Preto, University of São Paulo, Av. do Café, s/n, CEP 14040-904, Ribeirão Preto, São Paulo, Brazil.
Journal of Biomedical Materials Research. Part A
|February 22, 2007
Summary
Fully crystallized bioactive glass-ceramics, like Biosilicate, enhance in vitro osteogenesis. This biomaterial promotes greater calcified matrix formation, indicating improved bone-like tissue development for implants.
Area of Science:
- Biomaterials Science
- Biomineralization
- Tissue Engineering
Background:
- Bioactive glasses are crucial for load-bearing implants.
- Glass-ceramics offer improved mechanical properties.
- Biosilicate is a novel bioactive glass-ceramic.
Purpose of the Study:
- Evaluate Biosilicate's effect on in vitro osteogenesis.
- Assess Biosilicate's interaction with osteogenic cells.
- Determine Biosilicate's potential for bone regeneration.
Main Methods:
- Surface characterization using SEM and FTIR.
- In vitro culture of osteogenic cells on Biosilicate and control glasses.
- Analysis of apatite layer formation in simulated body fluid.
- Monitoring of cellular behavior and matrix deposition over 17 days.
Main Results:
- All tested materials formed apatite layers.
- Silica-gel and amorphous calcium phosphate layers formed rapidly.
- Biosilicate supported significantly larger areas of calcified matrix by day 17.
- Actin cytoskeleton dynamics varied with cell density and material interaction.
Conclusions:
- Full crystallization of bioactive glasses enhances in vitro bone-like tissue formation.
- Biosilicate shows promise for promoting osteogenesis in bone regeneration applications.
- The P2O5-Na2O-CaO-SiO2 system offers a viable route for developing advanced bone graft materials.
