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Updated: Jul 16, 2026

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images
Published on: September 7, 2017
Role of DNA methylation in stable gene repression
Laura Lande-Diner1, Jianmin Zhang, Ittai Ben-Porath
1Department of Cellular Biochemistry and Human Genetics, Hebrew University Medical School, Ein Kerem, 91120 Jerusalem, Israel.
Abstract:
A large fraction of the animal genome is maintained in a transcriptionally repressed state throughout development. By generating viable Dnmt1(-)(/)(-) mouse cells we have been able to study the effect of DNA methylation on both gene expression and chromatin structure. Our results confirm that the underlying methylation pattern has a profound effect on histone acetylation and is the major effector of me-H3(K4) in the animal genome. We demonstrate that many methylated genes are subject to additional repression mechanisms that also impact on histone acetylation, and the data suggest that late replication timing may play an important role in this process.
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