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Updated: Jul 16, 2026

Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
Platelet aggregation and adenosine triphosphate secretion after laparotomy
1Department of Obstetrics and Gynecology, Koshigaya Hospital, Dokkyo University School of Medicine, 2-1-50, Minami-Koshigaya, Koshigaya-shi, Saitama 343-8555, Japan. mhayashi@lilac.plala.or.jp
Abstract:
Live platelets were used to determine whether platelet count, volume, aggregation, and the amount of secreted adenosine triphosphate (ATP) change after laparotomy. Peripheral blood was collected from 32 gynecological patients before, one day, and two weeks after laparotomy. The platelet count was significantly lower (p < 0.01) in patients one day after laparotomy than before the operation. The mean platelet volume was significantly lower in patients two weeks after laparotomy than before (p < 0.01) and one day after laparotomy (p < 0.01). Platelet aggregation and ATP secretion were investigated using a lumiaggregometer. Platelet aggregation upon induction by 5 microM adenosine diphosphate (ADP) was significantly higher (p < 0.05) in patients two weeks after laparotomy than before laparotomy. Although the amount of secreted ATP upon induction by 5 microM ADP did not differ between before and after laparotomy, the amount of secreted ATP induced by 5 microg/mL collagen was significantly lower (p < 0.05) in patients one day after laparotomy than before the operation. This discrepancy may have been caused by a difference in the action of the two reagents.
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