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Related Experiment Video

Updated: Jul 16, 2026

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

Published on: November 1, 2018

Ptr ToxA interacts with a chloroplast-localized protein.

Viola A Manning¹, Linda K Hardison, Lynda M Ciuffetti

¹Department of Botany and Plant Pathology, Oregon State University, Corvallis 97331, USA.

Molecular Plant-Microbe Interactions : MPMI

|February 23, 2007

Summary

Pyrenophora tritici-repentis toxin A (ToxA) targets chloroplasts in wheat. This study identifies ToxA binding protein 1 (ToxABP1) as a key interaction partner within chloroplasts, crucial for understanding tan spot disease.

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Last Updated: Jul 16, 2026

Affinity Purification of Chloroplast Translocon Protein Complexes Using the TAP Tag

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Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves

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Studying Protein Import into Chloroplasts Using Protoplasts

Studying Protein Import into Chloroplasts Using Protoplasts

Published on: December 10, 2018

Area of Science:

Plant pathology
Molecular biology
Biochemistry

Background:

Tan spot, caused by Pyrenophora tritici-repentis, is a significant wheat disease.
Host-selective toxins, like Ptr ToxA (ToxA), are critical virulence factors.
ToxA is known to enter wheat mesophyll cells and localize to chloroplasts in susceptible cultivars.

Purpose of the Study:

To identify plant proteins that interact with the wheat tan spot toxin, ToxA.
To elucidate the molecular mechanisms underlying ToxA pathogenicity in wheat.

Main Methods:

Yeast two-hybrid screening was employed to identify ToxA interacting proteins.
ToxABP1 localization and expression were analyzed in wheat cultivars.

Main Results:

ToxA interacts with a novel chloroplast protein, ToxA binding protein 1 (ToxABP1).
ToxABP1 shares structural similarities with animal endocytosis proteins.
ToxABP1 is present in chloroplast membranes and stroma in both sensitive and insensitive wheat, and ToxA interacts with a ToxABP1 complex on the chloroplast membrane.

Conclusions:

ToxABP1 is a plant protein that interacts with ToxA within chloroplasts.
This interaction may be a key step in ToxA-mediated pathogenesis of tan spot disease in wheat.
Understanding the ToxA-ToxABP1 interaction can inform strategies for disease resistance.