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Related Concept Videos

2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved in...
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other axis.

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Symmetry-based constant-time homonuclear dipolar recoupling in solid state NMR.

Robert Tycko1

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, USA. robertty@mail.nih.gov

The Journal of Chemical Physics
|February 23, 2007
PubMed
Summary

A new constant-time dipolar recoupling pulse sequence, PITHIRDS-CT, enhances solid-state NMR structural studies. It improves measurements of distances and molecular conformations in peptides and biomaterials.

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Area of Science:

  • Solid-state Nuclear Magnetic Resonance (NMR) spectroscopy.
  • Materials science and structural biology.

Background:

  • Constant-time dipolar recoupling pulse sequences are crucial for solid-state NMR (ssNMR) structural studies.
  • These sequences offer robustness against radio-frequency pulse imperfections and nuclear spin relaxation.

Purpose of the Study:

  • To develop a novel approach for constructing constant-time homonuclear dipolar recoupling sequences.
  • To introduce and experimentally validate a new symmetry-based pulse sequence, PITHIRDS-CT.

Main Methods:

  • Utilizing symmetry properties of the dipole-dipole interaction Hamiltonian under cyclic displacements.
  • Applying the PITHIRDS-CT sequence in (13)C NMR experiments on amino acid powders and amyloid fibrils.
  • Conducting (15)N- and (13)C-detected ssNMR measurements on peptides.

Main Results:

  • PITHIRDS-CT demonstrated effectiveness in measuring intermolecular distances in solid samples.
  • The sequence proved useful for determining intramolecular distances and molecular conformations in peptides.
  • Specific utility shown for measuring backbone psi torsion angles in uniformly labeled peptides.

Conclusions:

  • PITHIRDS-CT is a valuable tool for enhancing structural investigations using solid-state NMR.
  • The sequence facilitates accurate measurements of both intermolecular and intramolecular distances.
  • It aids in detailed studies of molecular conformations, particularly in peptides.