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Updated: Jul 16, 2026

Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish
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Microarray-based analysis of fish egg quality after natural or controlled ovulation.

Emilie Bonnet1, Alexis Fostier, Julien Bobe

  • 1INRA, UR1037 SCRIBE, IFR140, Ouest-Genopole, 35000 Rennes, France. emilie.bonnet@rennes.inra.fr <emilie.bonnet@rennes.inra.fr>

BMC Genomics
|February 23, 2007
PubMed
Summary

Controlled ovulation in fish significantly alters egg gene expression, impacting developmental potential. Prohibitin 2 (PHB2) mRNA levels predict egg viability, offering insights into reproductive biotechnologies.

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Area of Science:

  • Aquaculture and Reproductive Biology
  • Genomics and Transcriptomics
  • Fish Biology

Background:

  • Fish egg quality is crucial for biotechnologies like nuclear transfer.
  • Hormonal or photoperiodic control of ovulation can cause egg quality defects.
  • The impact of ovulation control on the egg transcriptome is understudied.

Purpose of the Study:

  • To investigate the rainbow trout (Oncorhynchus mykiss) egg transcriptome after natural or controlled ovulation.
  • To identify genes affected by ovulation control methods.
  • To explore the relationship between maternal mRNA abundance and egg developmental potential.

Main Methods:

  • Utilized 9152-cDNA microarrays for genome-wide transcriptome analysis.
  • Employed real-time PCR to validate gene expression patterns.

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  • Compared gene expression profiles between naturally ovulated and artificially controlled (hormonal, photoperiodic) eggs.

    • Main Results:

    • Identified 26 differentially expressed genes, with 17 validated by real-time PCR.
    • Hormonal induction of ovulation significantly increased mRNA abundance of Apolipoprotein C1 (APOC1) and tyrosine protein kinase HCK.
    • Prohibitin 2 (PHB2) egg mRNA abundance showed a negative correlation with developmental success.

    Conclusions:

    • Ovulation control (hormonal or photoperiodic) demonstrably alters the fish egg transcriptome.
    • Prohibitin 2 (PHB2) mRNA levels serve as a biomarker for egg developmental potential.
    • Findings provide insights into ovulation control mechanisms and egg developmental failure.