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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Hybrid Zones02:29

Hybrid Zones

Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.Gene flow and natural selection are evolutionary mechanisms that shape the outcome of a hybrid zone. Gene flow...
In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

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Performing Custom MicroRNA Microarray Experiments
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Published on: October 28, 2011

Cross-species microarray hybridizations: a developing tool for studying species diversity.

Carmiya Bar-Or1, Henryk Czosnek, Hinanit Koltai

  • 1Department of Ornamental Horticulture, ARO Volcani Center, Bet Dagan, Israel.

Trends in Genetics : TIG
|February 23, 2007
PubMed
Summary

Cross-species hybridization (CSH) on DNA microarrays enables gene-expression profiling for diverse species. This review highlights recent advancements and strategies to improve CSH quality for evolutionary and ecological studies.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • Cross-species hybridization (CSH) on DNA microarrays is increasingly used for comparative genomics and gene-expression profiling.
  • CSH is valuable for closely related species and those lacking dedicated microarray platforms.
  • It remains a non-standard technique compared to species-specific hybridization.

Purpose of the Study:

  • To review recent developments in CSH for cDNA and oligomer microarray platforms.
  • To discuss factors affecting CSH quality and suggest improvement strategies.
  • To support the use of CSH in investigating species diversity.

Main Methods:

  • Review of recent literature on cross-species hybridization techniques.
  • Analysis of factors influencing CSH quality in cDNA and oligomer microarrays.
  • Identification of strategies for enhancing CSH experiment design and data analysis.

Main Results:

  • CSH offers a viable method for gene-expression profiling across species.
  • Key factors influencing CSH quality include platform choice, experimental design, and data analysis.
  • Specific strategies can significantly improve the reliability of CSH results.

Conclusions:

  • CSH is a powerful tool for comparative, evolutionary, and ecological studies.
  • Improvements in methodology can enhance the utility of CSH for understanding species diversity.
  • Further research and standardization can solidify CSH's role in genomics.