Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-resolution solid-state NMR structure of an anticancer agent.

Adam Lange1, Thomas Schupp, Frank Petersen

  • 1Department for NMR-based Structural Biology, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Chemmedchem
|February 23, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tocilizumab but not Siltuximab prevents systemic inflammation in a humanized mouse model.

Frontiers in immunology·2026
Same author

Trityl-Nitroxide Triradicals for Efficient High-Field Dynamic Nuclear Polarization.

Analytical chemistry·2026
Same author

Collecting Large Datasets of Unambiguous Structural Restraints for Protein Structure Determination by 4D Proton-Detected Solid-State NMR.

Chemphyschem : a European journal of chemical physics and physical chemistry·2026
Same author

Probing the Conformation of BamC and BamE in Native Bacterial Membranes Using Solid-State NMR Spectroscopy.

Journal of the American Chemical Society·2026
Same author

Targeting the Membrane-Embedded Rhomboid Protease GlpG: A Multimodal Strategy for Inhibitor Discovery and Mechanistic Insight.

Angewandte Chemie (International ed. in English)·2026
Same author

Functions of the cell wall polysaccharide schizophyllan during vegetative growth of <i>Schizophyllum commune</i>.

Cell surface (Amsterdam, Netherlands)·2026

Solid-state Nuclear Magnetic Resonance (NMR) rapidly determined the 3D structure of Epothilone B in a solid state. This breakthrough enables atomic-level studies of drug interactions in challenging environments.

Area of Science:

  • Biophysics
  • Structural Biology
  • Chemical Physics

Background:

  • Epothilone B is a potent microtubule-stabilizing agent with therapeutic potential.
  • Determining the high-resolution structure of Epothilone B in solid or insoluble states is challenging.
  • Understanding molecular interactions is crucial for drug development.

Purpose of the Study:

  • To demonstrate the utility of solid-state Nuclear Magnetic Resonance (NMR) for Epothilone B structure determination.
  • To analyze the 3D structure and intermolecular interactions of Epothilone B in the polycrystalline state.
  • To establish a foundation for studying ligand-microtubule interactions in noncrystalline environments.

Main Methods:

  • Solid-state NMR spectroscopy was employed to rapidly acquire structural data.

Related Experiment Videos

  • High-resolution 3D structural analysis was performed on polycrystalline Epothilone B.
  • Stereospecific assignments were derived from the structural data.

    • Main Results:

    • A high-resolution 3D structure of Epothilone B was determined in the polycrystalline state.
    • The determined solid-state NMR structures showed excellent agreement with an average heavy atom RMSD of 0.14 Å.
    • Insights into intermolecular interactions and their effect on ssNMR chemical-shift parameters were obtained.

    Conclusions:

    • Solid-state NMR is a powerful technique for rapid, high-resolution structural determination of molecules like Epothilone B in solid states.
    • The method provides stereospecific assignments and reveals details of intermolecular forces.
    • This approach opens new avenues for investigating molecular interactions in biologically relevant, noncrystalline, and insoluble systems at the atomic level.