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Primary immunization in the canine lung. Soluble antigen induces a localized response
D N Weissman1, D E Bice, B A Muggenburg
1Albuquerque Veterans Administration Medical Center, NM 87108.
The American Review of Respiratory Disease
|January 1, 1992
Summary
Primary immunization of dogs with soluble antigen in the lungs triggers local IgM and IgA antibody production. The IgG response, however, spreads to both vaccinated and unvaccinated lung areas.
Area of Science:
- Immunology
- Pulmonary Medicine
- Veterinary Science
Background:
- Particulate antigen instillation induces localized pulmonary antibody responses in dogs.
- The local antibody response to soluble antigen after primary immunization in the canine respiratory tract is not well understood.
Purpose of the Study:
- To characterize the local immune response in dogs following primary intrapulmonary immunization with soluble antigen.
- To investigate the immunoglobulin class-specific antibody distribution in the lungs after intralobar antigen instillation.
Main Methods:
- Five beagle dogs were immunized via bronchoscope with keyhole limpet hemocyanin (KLH) in one lung lobe and saline in the contralateral lobe.
- Blood and bronchoalveolar lavage (BAL) fluids were collected over three weeks to monitor specific immune responses.
- Immunoglobulin class-specific antibody levels and antibody-producing cells were analyzed.
Main Results:
- Primary intrapulmonary KLH immunization induced anti-KLH antibody responses in blood and BAL fluids from both immunized and control lung lobes.
- Specific IgM and IgA responses were significantly higher in the immunized lung lobes.
- Specific IgG levels and IgG-producing cells were similar in BAL fluids from both immunized and control lung lobes.
Conclusions:
- Primary intrapulmonary immunization with soluble antigen in dogs elicits a local IgM and IgA response.
- The IgG response following intrapulmonary soluble antigen instillation is distributed to both immunized and unimmunized lung regions.
- This differential immunoglobulin class-specific response pattern may influence regional lung immunity to intrapulmonary antigens.