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A panel of monoclonal antibodies recognizing the Staphylococcus epidermidis fibrinogen-binding MSCRAMM SdrG
Andrea E Hall1, Pratiksha R Patel, Paul J Domanski
1Inhibitex, Inc., Alpharetta, Georgia 30004, USA.
Abstract:
Staphylococcus epidermidis is an important opportunistic human pathogen that has recently emerged as a major cause of foreign-body infections. The most important stage contributing to the pathogenesis of this bacteria is the initial adherence to host tissue. SdrG is a cell-wall-anchored fibrinogen-binding adhesin of S. epidermidis that has been shown to be necessary for bacterial binding to fibrinogen-coated foreign bodies, such as catheters. Here we report the generation and characterization of a panel of monoclonal antibodies (MAbs) directed against this S. epidermidis virulence factor. Through the use of multiple in vitro assays, surface plasmon resonance, and flow cytometry, we have characterized a diverse array of MAbs that may prove to be beneficial in studies that address the precise biologic role of SdrG.
Insights
Researchers developed monoclonal antibodies (MAbs) targeting SdrG, a key adhesin in Staphylococcus epidermidis foreign-body infections. These MAbs aid in understanding SdrG's role in bacterial adherence and developing new treatments.
Area of Science:
- Microbiology
- Immunology
- Infectious Diseases
Background:
- Staphylococcus epidermidis is a significant opportunistic pathogen causing foreign-body infections.
- Bacterial adherence to host tissues is crucial for S. epidermidis pathogenesis.
- SdrG, a fibrinogen-binding adhesin, mediates bacterial attachment to foreign materials like catheters.
Purpose of the Study:
- To generate and characterize monoclonal antibodies (MAbs) against the S. epidermidis virulence factor SdrG.
- To investigate the potential of these MAbs in studying the biological role of SdrG.
Main Methods:
- Generation of a panel of monoclonal antibodies (MAbs).
- Characterization using in vitro assays, surface plasmon resonance (SPR), and flow cytometry.
Main Results:
- A diverse set of MAbs targeting SdrG was successfully generated and characterized.
- The MAbs demonstrated varied binding properties and specificities.
Conclusions:
- The developed MAbs provide valuable tools for further research into SdrG's function.
- These MAbs may contribute to understanding and combating S. epidermidis foreign-body infections.
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