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Related Experiment Videos

Single cell transfection using plasmid decorated AFM probes.

Charles M Cuerrier1, Réjean Lebel, Michel Grandbois

  • 1Département de pharmacologie, Faculté de Médecine, Université de Sherbrooke, Sherbrooke, Qué., Canada J1H 5N4.

Biochemical and Biophysical Research Communications
|February 24, 2007
PubMed
Summary

This study introduces a novel atomic force microscope (AFM) tip method for transfecting individual eukaryotic cells. The technique successfully delivers genetic material with minimal cell disturbance, offering a new tool for targeted gene delivery.

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Area of Science:

  • Cell Biology
  • Biophysics
  • Nanotechnology

Background:

  • Traditional cell transfection methods can be inefficient and cause significant cell damage.
  • Targeted delivery of genetic material to specific cells is crucial for various biological studies and therapeutic applications.

Purpose of the Study:

  • To develop and evaluate a novel method for transfecting individual eukaryotic cells using atomic force microscope (AFM) tips.
  • To assess the efficiency, cell survival, and growth following AFM-mediated gene delivery.

Main Methods:

  • Eukaryotic cells were individually transfected using AFM tips functionalized with plasmid DNA encoding enhanced green fluorescent protein (EGFP).
  • Cellular deformation was monitored during tip penetration to detect membrane passage.
  • Transfection success, cell viability, and growth were confirmed using epifluorescence microscopy.

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Main Results:

  • The AFM tip successfully penetrated the cell membrane, facilitating genetic material transfer.
  • A 30% transfection success rate was achieved with minimal cell disturbance.
  • Post-transfection cell survival and growth were confirmed, indicating minimal damage.

Conclusions:

  • AFM tip-mediated transfection offers a precise and minimally invasive method for targeting individual cells.
  • This technique expands the available tools for targeted gene delivery in eukaryotic cells.
  • The method demonstrates potential for applications requiring specific cell manipulation and genetic modification.