Related Experiment Video
Updated: Jul 16, 2026
Combined Recombinase Polymerase Amplification CRISPR/Cas12a Assay for Detecting Fusarium oxysporum f. sp. cubense Tropical Race 4
Published on: November 14, 2025
Sensitive and specific detection of Xanthomonas campestris pv. vesicatoria by PCR using pathovar-specific primers
Dong Suk Park1, Jae Kyung Shim, Jung Sun Kim
1National Institute of Agricultural Biotechnology, Rural Development Administration, 441-707 Suwon, Republic of Korea. dspark@rda.go.kr
Abstract:
The present study describes PCR assay to detect bacterial spot caused by Xanthomonas campestris pv. vesicatoria in pepper and tomato. One set of PCR primer was developed to amplify gene required for an rhs family gene homologous to rhsA, cell envelope biogenesis, outer membrane. Only a PCR product of a 517bp was produced in PCR reaction with the Xanthomonas campestris pv. vesicatoria (XCVF/XCVR) primer set. A specific, and highly sensitive and rapid PCR assay for the detection of X. campestris pv. vesicatoria was achieved. The protocol can be used as a reliable diagnostic tool for specific detection of X. campestris pv. vesicatoria in pepper or tomato.
