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Updated: Jul 16, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
Published on: May 29, 2019
DNA damage caused by pesticide-contaminated soil
K Krishnamurthi1, S Saravana Devi, T Chakrabarti
1Environmental Biotechnology Division, National Environmental Engineering Research Institute, Nehru Marg, Nagpur-440020, India. twmneeri@nagpur.dot.net.in
Objective:
To determine the DNA damaging potential and the genotoxicity of individual compounds in pesticide contaminated soil.
Methods:
In the present study, DNA damaging potential of pesticide-contaminated soil and the genotoxicity of individual compounds present in the soil were assessed using fluorimetric analysis of DNA unwinding assay.
Results:
The contaminated soil sample showed 79% (P < 0.001) of DNA strand break, whereas technical grade of major carbaryl and alpha-naphthol constituents of the contaminated soil showed 64% (P < 0.01) and 60% (P < 0.02) damage respectively.
Conclusion:
Our results indicate that the toxicity caused by contaminated soil is mainly due to carbaryl and alpha-napthol, which are the major constituents of the soil sample analyzed by GC-MS.
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