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Segregation of transgenes in maize.

T M Spencer¹, J V O'Brien, W G Start

¹Discovery Research, DEKALB Plant Genetics, Groton, CT 06340.

Plant Molecular Biology

|January 1, 1992

Summary

Gene expression and inheritance in transgenic maize were studied. While most genes segregated as expected, one line showed unstable gene transmission, impacting future research.

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Area of Science:

Plant biotechnology
Genetics
Molecular biology

Background:

Transgenic crops require stable gene integration and expression for successful application.
Understanding gene segregation and stability is crucial for breeding programs.

Purpose of the Study:

To analyze the segregation, expression, and stability of introduced genes in transgenic maize.
To assess the inheritance patterns of marker genes (bar and uidA) in subsequent generations.

Main Methods:

Analysis of progeny (R1 and R2) from backcrossed transgenic maize (R0).
Assessing phosphinothricin acetyltransferase (PAT) activity to confirm bar gene expression.
Molecular analysis to confirm gene segregation and cosegregation with uidA.

Main Results:

Segregation of the bar gene was observed in three out of four independent transformation events.
The bar and uidA genes showed genetic linkage in all analyzed populations.
One line exhibited unstable or poor transmission of integrated bar gene copies.

Conclusions:

The study demonstrates variable gene stability in transgenic maize lines.
Genetic linkage between marker genes is consistent across transformation events.
Further investigation is needed for lines with unstable gene transmission for reliable trait development.

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