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Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

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Related Experiment Video

Updated: Jul 13, 2026

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

Published on: November 21, 2015

The Intestinal Wnt/TCF Signature.

Laurens G Van der Flier¹, Jacob Sabates-Bellver, Irma Oving

¹Hubrecht Institute, Netherlands Institute for Developmental Biology and Centre for Biomedical Genetics, Utrecht, The Netherlands.

Gastroenterology

|February 27, 2007

Summary

Activating Wnt pathway mutations in colorectal cancer drive cell transformation via a specific gene program. This intestinal Wnt/TCF signature gene set is crucial for tumor development and originates from stem cell compartments.

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Last Updated: Jul 13, 2026

Protocols for Analyzing the Role of Paneth Cells in Regenerating the Murine Intestine using Conditional Cre-lox Mouse Models

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Published on: November 21, 2015

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Published on: April 1, 2021

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Colorectal cancer involves Wnt pathway activation, leading to epithelial cell transformation.
This transformation is driven by aberrant expression of TCF4 target genes normally found in intestinal crypts.

Purpose of the Study:

To comprehensively analyze the Wnt/TCF target gene program in colorectal cancer.
To identify the core gene set responsible for intestinal epithelial cell transformation.

Main Methods:

Array-based analysis of Wnt/TCF target genes in colorectal cancer cell lines with inducible Wnt cascade blockade.
Differential gene expression profiling of human adenomas, adenocarcinomas, and normal colonic epithelium.
Expression analysis of common genes in a murine adenoma model.

Main Results:

Identification of the intestinal Wnt/TCF signature gene set, responsible for human intestinal epithelial cell transformation.
This core gene program is consistently expressed in adenomas.
Genes could be categorized into 3 modules based on distinct crypt compartment expression.

Conclusions:

A module of 17 genes was specifically identified at the crypt stem cell location.
The intestinal Wnt/TCF signature gene set plays a critical role in colorectal cancer development.