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Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Crossing Over01:30

Crossing Over

Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...

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Related Experiment Video

Updated: Jul 16, 2026

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
08:44

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

Published on: October 23, 2014

The INO80 complex is required for damage-induced recombination.

Satoshi Kawashima1, Hideaki Ogiwara, Shusuke Tada

  • 1Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Aoba 6-3, Aramaki, Aoba-ku, Sendai 980-8578, Japan.

Biochemical and Biophysical Research Communications
|February 27, 2007
PubMed
Summary

The INO80 chromatin remodeling complex facilitates DNA repair through homologous recombination. Its component Arp8 is crucial for this process, while the related SWR1 complex does not promote recombination.

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Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay
13:10

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Area of Science:

  • Molecular Biology
  • Genetics
  • Chromatin Biology

Background:

  • The INO80 and SWR1 complexes are involved in chromatin remodeling and share components like Arp4.
  • These complexes are recruited to DNA double-strand break (DSB) sites.
  • INO80 can remodel nucleosomes near DSBs but its role in repair is unclear.

Purpose of the Study:

  • To investigate the role of the INO80 complex and its components in DNA repair pathways.
  • To determine if chromatin remodeling by INO80 facilitates homologous recombination after DNA damage.

Main Methods:

  • Analysis of recombination frequencies in budding yeast mutants.
  • Investigating the function of Arp8 and Arp6 in DNA repair.
  • Assessing the role of the INO80 and SWR1 complexes in response to DNA damage.

Main Results:

  • Arp8, an essential factor for INO80 activity, is involved in damage-induced sister chromatid and interchromosomal recombination.
  • Mutants in arp6, a component of the SWR1 complex, show proficient recombination.
  • The SWR1 complex does not appear to promote recombination.

Conclusions:

  • Chromatin remodeling by the INO80 complex is essential for efficient homologous recombination following DNA damage.
  • Arp8 plays a critical role in facilitating DNA repair through homologous recombination.
  • The SWR1 complex's function in recombination differs from that of the INO80 complex.