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Related Experiment Video

Updated: Jul 16, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Efficient transgenic rat production by a lentiviral vector.

Mieczyslaw Michalkiewicz1, Teresa Michalkiewicz, Aron M Geurts

  • 1Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd., Milwaukee, WI 53226, USA. mmichalk@mcw.edu

American Journal of Physiology. Heart and Circulatory Physiology
|February 27, 2007
PubMed
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Lentiviral transgenesis efficiently created transgenic rats with high green fluorescent protein (GFP) expression. This method is powerful for gene discovery and validating cardiovascular targets in rats.

Area of Science:

  • Genetics
  • Molecular Biology
  • Animal Models

Background:

  • Developing transgenic rat models is crucial for gene function studies.
  • Lentiviral vectors offer a promising approach for creating genetically modified organisms.

Purpose of the Study:

  • To evaluate the utility of lentiviral transgenesis for generating transgenic rat lines for gene function studies.
  • To assess the efficiency and characteristics of transgene integration and expression.

Main Methods:

  • Microinjection of a lentiviral construct encoding enhanced green fluorescent protein (eGFP) driven by the CAG promoter into rat embryos.
  • Generation and characterization of transgenic rat lines from Dahl S and Sprague-Dawley strains.
  • Analysis of transgene copy number, insertion sites, and eGFP expression patterns.

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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
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Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
08:46

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

Related Experiment Videos

Last Updated: Jul 16, 2026

Generation of Transgenic Rats using a Lentiviral Vector Approach
09:07

Generation of Transgenic Rats using a Lentiviral Vector Approach

Published on: May 17, 2020

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
10:21

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

Published on: October 7, 2018

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
08:46

Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

Published on: May 24, 2012

Main Results:

  • High birth rates (49.6%) and successful generation of transgenic rats with rates of 22% (Dahl S) and 14% (Sprague-Dawley).
  • Transgenes integrated as single copies, predominantly in introns, with low copy numbers (1-4) in founders.
  • Consistent high eGFP expression in key organs like brain, kidney, heart, and vasculature across different transgenic lines, independent of chromosomal insertion site.

Conclusions:

  • Lentiviral transgenesis is an effective tool for producing transgenic inbred rats.
  • The CAG promoter drives robust eGFP expression, suitable for cardiovascular research and gene discovery.
  • This approach significantly enhances the utility of rats in genetic research and target validation.