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Related Experiment Video

Updated: Jul 16, 2026

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
07:06

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model

Published on: October 10, 2025

Tissue engineered intervertebral disc repair in the pig using injectable polymers.

P A Revell1, E Damien, L Di Silvio

  • 1Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, London WC1X 8LD, UK. parevell@btinternet.com

Journal of Materials Science. Materials in Medicine
|February 27, 2007
PubMed
Summary

Tissue engineering using HYAFF 120 and HYADD 3 successfully restored intervertebral disc (IVD) structure and cellularity after nucleotomy in pigs. These hyaluronan-derived materials offer promising solutions for disc degeneration.

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Surgery

Background:

  • The intervertebral disc (IVD) comprises a nucleus pulposus (NP) for compression resistance and an annulus fibrosus for strength.
  • Tissue engineering of IVD substitutes faces mechanical and biological challenges, including nutrient transport.
  • Nucleotomy, a procedure involving removal of disc material, leads to structural degradation.

Purpose of the Study:

  • To evaluate the efficacy of two hyaluronan-derived polymeric materials, HYAFF 120 and HYADD 3, as injectable nucleus pulposus substitutes.
  • To assess the potential of HYADD 3 when combined with bone marrow stem cells for IVD repair.
  • To investigate the histopathological outcomes of these treatments in a porcine lumbar spine model.

Main Methods:

Related Experiment Videos

Last Updated: Jul 16, 2026

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model
07:06

Surgical Technique for the Implantation of a Biomimetic Artificial Intervertebral Disc in a Goat Animal Model

Published on: October 10, 2025

  • Female pigs underwent nucleotomy in lumbar discs, followed by injection of HYAFF 120, HYADD 3, or HYADD 3 with bone marrow stem cells.
  • Control groups received nucleotomy only.
  • Lumbar spines were harvested after 6 weeks for histopathological analysis.

    • Main Results:

    • Nucleotomy alone caused significant IVD structural loss, including narrowing and fibrous tissue replacement.
    • Both HYAFF 120 and HYADD 3 treatments effectively prevented the degenerative changes observed in control discs.
    • Treated discs exhibited NP-like regions with viable chondrocytes producing matrix similar to normal IVD tissue.

    Conclusions:

    • Hyaluronan-derived materials HYAFF 120 and HYADD 3 demonstrate potential as injectable substitutes for nucleus pulposus.
    • These biomaterials can preserve IVD structure and promote the formation of NP-like tissue post-nucleotomy.
    • Further research into cell-seeded hydrogels may enhance regenerative capacity for treating disc degeneration.