Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantifying tumour-infiltrating lymphocyte subsets: a practical immuno-histochemical method.

Paula M Loughlin1, Timothy G Cooke, W David George

  • 1Section of Surgical Sciences and Translational Research, University of Glasgow, UK.

Journal of Immunological Methods
|February 28, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gluten Induces Subtle Histological Changes in Duodenal Mucosa of Patients with Non-Coeliac Gluten Sensitivity: A Multicentre Study.

Nutrients·2022
Same author

Worldviews.

International psychiatry : bulletin of the Board of International Affairs of the Royal College of Psychiatrists·2019
Same author

A single nucleotide polymorphism in codon F31I and V57I of the AURKA gene in invasive ductal breast carcinoma in Middle East.

Medicine·2017
Same author

ROC-king onwards: intraepithelial lymphocyte counts, distribution & role in coeliac disease mucosal interpretation.

Gut·2017
Same author

The Pretreatment Systemic Inflammatory Response is an Important Determinant of Poor Pathologic Response for Patients Undergoing Neoadjuvant Therapy for Rectal Cancer.

Annals of surgical oncology·2016
Same author

The gastric acid pocket is attenuated in <i>H. pylori</i> infected subjects.

Gut·2016
Same journal

Optimized intracellular flow cytometry panel enables CD4 and CD8 T cell cytokine profiling in Syrian hamsters.

Journal of immunological methods·2026
Same journal

Isosulfan blue sentinel lymph node biopsy enables reliable lymph node harvest and multicolor flow cytometry in mice.

Journal of immunological methods·2026
Same journal

Type-specific antibody detection of herpes simplex virus types 1&2 (HSV-1&2) in fingerstick blood at point-of-care sites by a rapid and sensitive lateral flow immunochromatographic assay.

Journal of immunological methods·2026
Same journal

Development of chimeric DGP-IgG antibodies as quality control for celiac disease diagnosis.

Journal of immunological methods·2026
Same journal

Stepwise single-cell-resolved deep immunophenotyping pipeline to characterise immune heterogeneity and functionality in health and disease.

Journal of immunological methods·2026
Same journal

Performance evaluation of Meso Scale Discovery (MSD) quantitative serological assays for detection of binding (IgG, IgA, IgM) and ACE2 inhibitory antibody levels for SARS-CoV-2.

Journal of immunological methods·2026
See all related articles

This study presents an efficient method for quantifying tumour-infiltrating lymphocyte (TIL) subsets in breast cancer tissue. The developed technique uses common software for accurate and precise histological analysis of TIL density.

Area of Science:

  • Immunohistochemistry
  • Digital Pathology
  • Quantitative Biology

Background:

  • Accurate quantification of tumour-infiltrating lymphocytes (TILs) is crucial for understanding their role in human cancer.
  • Existing methods for TIL subset analysis in archival tissues can be inefficient.
  • Developing a streamlined histological quantification method is needed.

Purpose of the Study:

  • To develop an efficient and accurate method for quantifying T and B lymphocyte (TIL) subsets in archival breast cancer tissues.
  • To establish a reliable histological quantification technique for TIL density.

Main Methods:

  • Acquired ten x40 digital images from 16 ductal invasive breast carcinoma microsections stained for CD3, CD4, CD8, and CD20.
  • Utilized Adobe Photoshop 7 'Color range' and 'Histogram' tools to count pixels corresponding to immunostained cells.

Related Experiment Videos

  • Converted pixel counts to cell counts per mm(2) using a calibration factor derived from image analysis.

    • Main Results:

    • Individual calibration per case/antibody combination was necessary due to variations in labelled pixels per cell.
    • Calibration using two fields yielded cell counts minimally higher (+5.3%) than ten-field calibration, with acceptable confidence limits (-14.7 to +25.3%).
    • The achieved accuracy and precision are suitable given the potential 100-fold variation in TIL density between cases.

    Conclusions:

    • The described methodology provides sufficient accuracy, precision, and efficiency for quantifying TIL sub-populations in breast cancer.
    • This method employs commonly available software and can be adapted for batch processing of image files.
    • The technique facilitates investigations into the role of TILs in cancer biology using archival specimens.