Related Experiment Video
Updated: Jul 16, 2026
Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays
Published on: April 4, 2011
Reliable universal RT-PCR assays for studying influenza polymerase subunit gene sequences from all 16 haemagglutinin
Olive T W Li1, Ian Barr, Connie Y H Leung
1State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China.
Abstract:
Realizable one-step RT-PCR assays specific for influenza PB2, PB1 and PA segments are described in this report. The designs of the consensus primers were based on more than five thousands polymerase genes derived from avian or mammalian viral strains. All the viral RNA tested in this study could be consistently amplified by the assays. The reaction products were specific and could be used for direct DNA sequencing. These assays might be useful tools to study the sequences of these genes.
