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Related Experiment Video

Updated: Jul 16, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

Quantal release of ATP in mouse cortex.

Yuriy Pankratov1, Ulyana Lalo, Alexei Verkhratsky

  • 1Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.

The Journal of General Physiology
|February 28, 2007
PubMed
Summary

Cortical neurons exhibit spontaneous currents, with smaller events likely reflecting ATP release alongside glutamate. This ATP release, from a subset of terminals, occurs less frequently than glutamate release.

Area of Science:

  • Neuroscience
  • Neurophysiology
  • Molecular Biology

Background:

  • Cortical neurons exhibit spontaneous transient currents reflecting quantal neurotransmitter release.
  • Key neurotransmitters include glutamate and gamma-aminobutyric acid (GABA).

Purpose of the Study:

  • To investigate the origin and characteristics of spontaneous inward currents in mouse pyramidal neurons.
  • To differentiate the contributions of various neurotransmitters and receptors to these currents.

Main Methods:

  • Recording spontaneous inward currents from mouse pyramidal neurons in acute brain slices.
  • Utilizing receptor antagonists (glutamate and P2X receptors) and pharmacological modulators (cyclothiazide).
  • Stimulating single intracortical axons to evoke quantal currents.

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Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
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Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Published on: December 19, 2019

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons
06:54

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Published on: September 9, 2020

Related Experiment Videos

Last Updated: Jul 16, 2026

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice
08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Published on: March 11, 2020

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
11:20

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK

Published on: December 19, 2019

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons
06:54

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

Published on: September 9, 2020

Main Results:

  • A bimodal amplitude distribution of spontaneous inward currents was observed.
  • Larger currents were sensitive to glutamate receptor antagonists (AMPA, kainate).
  • Smaller currents were partially inhibited by P2X receptor antagonists and mimicked by axonal stimulation, suggesting ATP release.

Conclusions:

  • Spontaneous currents in cortical neurons originate from both glutamate and ATP release.
  • ATP is released in packets from a subset of glutamate-containing terminals with lower probability than glutamate.
  • These findings reveal a novel role for ATP as a co-released neurotransmitter in the cortex.