Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrochemically addressable functionalization and parallel readout of a DNA biosensor array.

Lifang Niu1, Wolfgang Knoll

  • 1Institute of Materials Research & Engineering, 3 Research Link, Singapore.

Analytical Chemistry
|March 1, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing doubled haploid technology for alfalfa breeding.

Fundamental research·2026
Same author

The F-Box Protein DWARF1 Controls Plant Height by Modulating MtSMXL6 and MtSMXL7 Stability in Medicago truncatula.

Plant, cell & environment·2026
Same author

In Vivo Maternal Haploid Induction by Disrupting KOKOPELLI in Medicago truncatula.

Plant biotechnology journal·2026
Same author

SPARC: A programmable molecular diagnostic platform based on a signal-triggered, self-supplied crRNA and tiered PER-transcription-CRISPR cascade for early detection of hepatocellular carcinoma.

Analytica chimica acta·2026
Same author

Creating inbred lines for heterosis utilization in alfalfa.

Science China. Life sciences·2025
Same author

An Aptamer-Based gFET-Sensor for Specific Quantification of Gene Therapeutic Human Adenovirus Type 5.

Biosensors·2025
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

We developed an electrochemical method for assembling DNA capture probes on electrode arrays. This technique enables real-time monitoring of DNA hybridization, advancing DNA array fabrication and analysis.

Area of Science:

  • Electrochemistry
  • Biotechnology
  • Analytical Chemistry

Background:

  • Fabricating DNA arrays for biological sensing is challenging.
  • Current methods for probe assembly and hybridization detection can be complex and time-consuming.

Purpose of the Study:

  • To introduce a novel electrochemical method for directed in situ assembly of oligonucleotide probes.
  • To enable on-line, quantitative monitoring of parallel hybridization reactions.

Main Methods:

  • Utilized an electrochemical method for in situ probe assembly on a multielectrode array.
  • Employed surface plasmon microscopy for real-time monitoring of functionalization.
  • Used surface plasmon field-enhanced fluorescence microscopy for hybridization detection with organic dyes or quantum dots.

Related Experiment Videos

Main Results:

  • Successfully demonstrated directed in situ assembly of specific oligonucleotide probes.
  • Achieved on-line recording of probe functionalization and hybridization events.
  • Showcased color-multiplexing capabilities using different labels for target detection.

Conclusions:

  • This electrochemical approach offers a new strategy for fabricating DNA arrays.
  • The method allows for efficient and quantitative evaluation of parallel hybridization reactions in a flow cell environment.