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Updated: Jul 16, 2026

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Application of Laser Micro-irradiation for Examination of Single and Double Strand Break Repair in Mammalian Cells

Published on: September 5, 2017

Development of a nanotechnology based low-LET multi-microbeam array single cell irradiation system.

S Chang¹, J Zhang, D Bordelon

¹Department of Radiation Oncology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. sha_chang@med.unc.edu

Radiation Protection Dosimetry

|March 1, 2007

Summary

A new carbon nanotube (CNT) system enables precise, high-throughput single cell irradiation. This novel technology allows for simultaneous irradiation of many selected cells with an electron microbeam.

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Area of Science:

Biophysics
Materials Science
Nanotechnology

Background:

Single cell irradiation is crucial for understanding cellular responses to radiation.
Existing microbeam systems often lack the precision and throughput for large-scale studies.
Carbon nanotube (CNT) field emission technology offers potential for advanced electron beam generation.

Purpose of the Study:

To introduce a novel single cell irradiation system utilizing carbon nanotube (CNT) based field emission technology.
To demonstrate the capability of producing a high-resolution electron microbeam for precise cellular targeting.
To report preliminary development results of a multipixel microbeam array system.

Main Methods:

Development of a CNT-based field emission electron source.

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Laser Micro-Irradiation to Study DNA Recruitment During S Phase

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Integration of a multipixel array for individually controlled microbeams.

Utilizing microscope imaging for real-time cell targeting and coordinate identification.

Characterization of electron microbeam properties (energy, pulsation frequencies, dose rates).

Main Results:

Successful development of a prototype single pixel CNT microbeam device.
Demonstration of electron microbeam generation with energies between 20 and 60 keV.
Capability for producing microbeams with variable pulsation frequencies and dose rates.
Design enabling up to 10,000 individually controlled microbeam pixels, each ~10 micrometers in size.

Conclusions:

The developed CNT-based system represents a significant advancement in single cell irradiation technology.
The multipixel architecture allows for unprecedented throughput and precision in cellular irradiation experiments.
This technology holds promise for simultaneous irradiation of multiple selected cells under real-time microscopic observation.