Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

His-tag impact on structure.

Mike Carson1, David H Johnson, Heather McDonald

  • 1Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 251 CBSE, 1025 18th Street South, Birmingham, AL 35294-4400, USA. carson@uab.edu

Acta Crystallographica. Section D, Biological Crystallography
|March 1, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cholesterol differentially regulates α-synuclein binding across membrane packing regimes.

bioRxiv : the preprint server for biology·2026
Same author

The moderating role of anxiety sensitivity in the association between momentary PTSD symptoms and suicide cognitions.

Behaviour research and therapy·2026
Same author

Membrane Phase, Charge, and Curvature Regulate α-Synuclein Binding Dynamics.

bioRxiv : the preprint server for biology·2026
Same author

Prevalence of intraretinal and subretinal fluid in acute non-arteritic anterior ischemic optic neuropathy.

Eye (London, England)·2026
Same author

Recombinant protein platform for high-throughput investigation of peptide-liposome interactions via fluorescence anisotropy depolarization.

Communications chemistry·2026
Same author

The disordered and structured regions of <math><mi>α</mi></math> -Synuclein contribute to membrane remodeling synergistically.

bioRxiv : the preprint server for biology·2026
Same journal

Structural insights into the synthesis of FMN in prokaryotic organisms.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Native sulfur/chlorine SAD phasing for serial femtosecond crystallography.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Serial crystallographic analysis of protein isomorphous replacement data from a mixture of native and derivative microcrystals.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

The first crystal structure of the peptidase domain of the U32 peptidase family.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Atomic resolution crystal structure of Sapp2p, a secreted aspartic protease from Candida parapsilosis.

Acta crystallographica. Section D, Biological crystallography·2015
Same journal

Structural characterization of a mitochondrial 3-ketoacyl-CoA (T1)-like thiolase from Mycobacterium smegmatis.

Acta crystallographica. Section D, Biological crystallography·2015
See all related articles

His tags generally do not affect protein structure, though higher resolution is seen when tags are resolved in crystal structures. Further PDB annotation is recommended.

Area of Science:

  • Structural biology
  • Biochemistry
  • Protein crystallography

Background:

  • Protein constructs often include His tags for purification.
  • The impact of His tags on native protein structure is often assumed to be negligible.

Purpose of the Study:

  • To survey and compare crystal structures with and without His tags.
  • To assess the effect of His tags on protein structure and crystallographic data.

Main Methods:

  • Comparative analysis of PDB crystal structures.
  • Examination of refined tag residues within electron density.
  • Assessment of resolution, R factors, and B factors.

Main Results:

  • His tag residues are refined into density in less than 10% of tagged structures.

Related Experiment Videos

  • Higher resolution crystals are observed when His tags are resolved.
  • No significant effect on native protein structure, resolution, or R factors.
  • Slightly higher overall B factors observed in tagged structures.

    • Conclusions:

    • His tags generally do not significantly alter the native protein structure.
    • The presence of resolved His tags correlates with improved crystal resolution.
    • Explicit annotation of His tags in PDB format is recommended for clarity.