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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Updated: Jul 16, 2026

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes

Published on: December 28, 2011

Transcriptome analysis of rat kidney cells continuously exposed to cadmium using DNA microarray.

Ken-ichi Ohba¹, Youhei Okawa, Yuki Matsumoto

¹Department of Health Administration, School of Allied Health Sciences, Kitasato University, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan. kohba@kitasato-u.ac.jp

The Journal of Toxicological Sciences

|March 1, 2007

Summary

This study investigated how rat kidney cells respond to cadmium exposure. Cadmium significantly altered gene expression, increasing 27 genes and decreasing 4 genes.

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Area of Science:

Toxicology
Molecular Biology
Genomics

Background:

Cadmium is a toxic heavy metal with known adverse effects on kidney function.
Understanding cellular responses to cadmium is crucial for assessing its health risks.

Purpose of the Study:

To investigate the transcriptional changes in rat kidney cells upon continuous cadmium exposure.
To identify specific genes affected by cadmium toxicity at the molecular level.

Main Methods:

Utilized DNA microarray analysis to assess gene expression profiles.
Maintained continuous exposure of rat kidney cells to cadmium.

Main Results:

Identified 27 genes with increased expression, including metallothioneins (Mt1, Mt2), glutathione S-transferase alpha 3 (GSTa3), and beta-2-microglobulin (B2m).
Observed a decrease in the expression of 4 genes.

Conclusions:

Continuous cadmium exposure induces significant alterations in the kidney cell transcriptome.
The identified genes may play a role in the cellular defense or damage mechanisms against cadmium toxicity.