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Updated: Jul 16, 2026
09:25
Establishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma
Published on: October 14, 2016
Survivin as a prognostic factor for osteosarcoma patients
Eiji Osaka1, Takashi Suzuki, Shunzo Osaka
1Department of Orthopaedic Surgery, Nihon University School of Medicine, 30-1 Oyaguchikamimachi, Itabashi-ku, Tokyo 173-8610, Japan. eosaka@med.nihon-u.ac.jp
Acta Histochemica Et Cytochemica
|March 1, 2007
Summary
High survivin mRNA expression in osteosarcoma tissues is linked to metastasis and poor prognosis. This finding suggests survivin mRNA levels could serve as a prognostic biomarker for osteosarcoma patients.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Genetics
Background:
- Survivin is an apoptosis inhibitor gene with limited expression in adult tissues.
- Elevated survivin expression is observed in various human cancers and correlates with prognosis.
- The role of survivin in osteosarcoma progression and patient outcomes requires further investigation.
Purpose of the Study:
- To investigate the correlation between survivin mRNA expression levels in osteosarcoma tissues and clinicopathological parameters.
- To determine if survivin mRNA expression can serve as a prognostic indicator in osteosarcoma.
Main Methods:
- Analysis of 22 osteosarcoma patient biopsy specimens.
- Extraction of total RNA and subsequent RT-PCR for survivin mRNA detection.
- Densitometric analysis of survivin mRNA relative to h-GAPDH as an internal control.
Main Results:
- Survivin mRNA expression was detected in all analyzed osteosarcoma samples.
- Patients with metastasis exhibited significantly higher survivin mRNA levels in initial biopsies (p<0.01).
- A statistically significant difference in survivin mRNA expression was observed between metastatic and non-metastatic patients (p<0.01).
Conclusions:
- High survivin mRNA expression levels in osteosarcoma tissues are associated with a poor prognosis.
- Survivin mRNA expression may function as a valuable biomarker for predicting outcomes in osteosarcoma patients.
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