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Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...

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Hexosome and hexagonal phases mediated by hydration and polymeric stabilizer.

Idit Amar-Yuli1, Ellen Wachtel, Einav Ben Shoshan

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Controlling polymer concentration is key to tuning the size, structure, and stability of GMO/tricaprylin/water hexosomes. Higher concentrations yield smaller, more stable hexosomes by balancing hydration and polymer adsorption.

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Area of Science:

  • Materials Science
  • Colloid Science
  • Nanotechnology

Background:

  • Hexosomes are self-assembled nanostructures formed from lipids and water.
  • Controlling hexosome properties is crucial for their application in various fields.
  • Pluronic 127 is a common stabilizer for soft particles.

Purpose of the Study:

  • To investigate factors controlling the formation and inner structure of GMO/tricaprylin/water hexosomes.
  • To understand the role of Pluronic 127 in hexosome stabilization.
  • To demonstrate tunability of hexosome properties via composition, polymer concentration, and processing.

Main Methods:

  • Small-angle X-ray scattering (SAXS) for morphology and inner structure.
  • Cryo-transmission electron microscopy (cryo-TEM) for detailed structural analysis.
  • Dynamic light scattering (DLS) and LUMiFuge for size and physical stability.
  • Varying internal composition, polymer concentration, and processing conditions.

Main Results:

  • Hexosome properties (size, structure, stability) are tunable by internal composition, polymer concentration, and processing.
  • Two competing processes govern hexosome formation: water penetration (hydration) and polymer incorporation (stabilization).
  • Higher Pluronic 127 concentrations (1-1.6 wt%) favor faster hydration over polymer adsorption, leading to smaller, more symmetric, and stable hexosomes.
  • Lower Pluronic 127 concentrations (<1.0 wt%) result in insufficient polymer adsorption, leading to larger, less ordered, and less stable hexosomes.

Conclusions:

  • The balance between lipid hydration and polymer adsorption dictates hexosome characteristics.
  • Optimal Pluronic 127 concentration is critical for achieving desired hexosome properties.
  • This study provides insights for precise control over hexosome formation for tailored applications.