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qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
Optimizing PCR positivity criterion for detection of herpes simplex virus DNA on skin and mucosa
Amalia S Magaret1, Anna Wald, Meei-Li Huang
1Department of Laboratory Medicine, University of Washington, 600 Broadway, Suite 400, Seattle, WA 98122, USA. amag@u.washington.edu
Journal of Clinical Microbiology
|March 3, 2007
Abstract:
In 1997, we developed a PCR assay for the detection of herpes simplex virus (HSV) DNA. Recently, we determined an optimal positivity criterion based on research specimens and a dilution study. We found that a cutoff of 50 HSV DNA copies/ml of swab specimen, a level 10-fold lower than our previous cutoff, minimizes misclassification.
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