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Related Experiment Video

Updated: Jul 16, 2026

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
08:56

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Published on: January 11, 2012

Semi-artificial Fluorescent Molecular Machine for DNA Damage Detection.

Vladimir V Didenko1, Candace L Minchew, Stewart Shuman

  • 1Baylor College of Medicine, Houston, Texas, Michael E. DeBakey Veterans Affairs Medical Center, Houston, Texas, and Sloan-Kettering Institute, New York, New York.

Nano Letters
|March 3, 2007
PubMed
Summary

Researchers created a novel semi-artificial molecular device by combining a natural protein with an artificial component. This innovative sensor can selectively detect various DNA breaks, paving the way for new molecular device designs.

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Area of Science:

  • Biochemistry
  • Nanotechnology
  • Molecular Engineering

Background:

  • Designing artificial molecular machines is challenging due to the incompatibility of macroscale mechanics with nanoscale environments.
  • Natural molecular machines offer complex functionalities but require integration with artificial components for tailored applications.

Purpose of the Study:

  • To engineer a novel semi-artificial molecular device for detecting DNA breaks.
  • To demonstrate a practical approach for integrating natural and artificial components in molecular device design.

Main Methods:

  • Constructed a semi-artificial molecular device by linking a naturally occurring vaccinia virus-encoded protein with an artificial component.
  • Incorporated two fluorescently labeled detector units into the self-assembled construct.

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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
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Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

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Last Updated: Jul 16, 2026

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
08:56

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage

Published on: January 11, 2012

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition
07:16

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)
13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Published on: September 24, 2015

Main Results:

  • The developed device is the first sensor capable of selectively detecting different types of DNA breaks.
  • The semi-artificial construct demonstrated successful self-assembly and functionality.

Conclusions:

  • This work presents a viable strategy for the design of sophisticated molecular devices.
  • The developed sensor offers a new tool for studying DNA damage and repair mechanisms.