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A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica
Published on: February 20, 2012
[Molecular ecology of Gardenia jasminoides authenticity]
Jianping Han1, Shilin Chen, Wen-Sheng Zhang
1Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China. jphan@implad.ac.cn
Abstract:
By using amplified fragment length polymorphism (AFLP) markers, this paper studied the genetic relationships among five wild or cultivated Gardenia jasminoides Ellis populations in Jiangxi Province. Chemical fingerprint was also built with HPLC method. The results showed that there was a great genetic difference among these samples. The UPGMA obtained with NTSYS-PC 2. 10e software suggested that there were seven branches of population, and the population from near geographical location clustered firstly. The geniposide content of these branches was not correlated with UPGMA. It could be concluded that the authenticity was resulted from the co-action of genotype and environmental change. The microelements content in G. jasminoides fruit measured by inductively coupled plasma showed that there was a negative correlation between Zn and geniposide contents.
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