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Related Concept Videos

Malaria01:29

Malaria

Malaria pathogenesis in humans reflects a delicate interplay between parasite biology and host response. Clinical illness reflects a host’s immune response to the parasite’s asexual replication cycle, which is often asymptomatic in individuals with partial immunity. From the parasite's perspective, transmission between mosquito and human with minimal host pathology is evolutionarily advantageous. Among the six Plasmodium species infecting humans, P. falciparum and P. vivax dominate in global...
Symbiosis00:58

Symbiosis

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...
The Blood-brain Barrier00:49

The Blood-brain Barrier

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Related Experiment Video

Updated: Jul 16, 2026

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells
10:09

Selection of Plasmodium falciparum Parasites for Cytoadhesion to Human Brain Endothelial Cells

Published on: January 3, 2012

Plasmodium falciparum-infected erythrocytes decrease the integrity of human blood-brain barrier endothelial cell

Abhai K Tripathi1, David J Sullivan, Monique F Stins

  • 1W. Harry Feinstone Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.

The Journal of Infectious Diseases
|March 3, 2007
PubMed
Summary

Plasmodium falciparum-infected red blood cells (Pf-IRBCs) disrupt the blood-brain barrier (BBB) integrity in cerebral malaria. Both membrane and soluble Pf-IRBC factors contribute to this BBB dysfunction.

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Published on: April 5, 2015

Area of Science:

  • Neuroscience
  • Infectious Disease Research
  • Cell Biology

Background:

  • Cerebral malaria (CM) pathogenesis involves Plasmodium falciparum-infected red blood cells (Pf-IRBCs) sequestering at the blood-brain barrier (BBB).
  • Molecular mechanisms linking Pf-IRBCs to BBB dysfunction remain poorly understood.

Purpose of the Study:

  • To investigate how Pf-IRBCs affect the integrity of the human BBB.
  • To identify Pf-IRBC components responsible for BBB disruption.

Main Methods:

  • Utilized an in vitro human BBB model with electrical cell substrate sensing and transendothelial electrical resistance measurements.
  • Subfractionated Pf-IRBCs and treated them with trypsin, artemisinin, or brefeldin A.

Main Results:

  • Pf-IRBCs significantly reduced BBB electrical resistance, unlike normal red blood cells.
  • Both membrane-associated and soluble Pf-IRBC factors were found to decrease BBB resistance.
  • Trypsin treatment reduced Pf-IRBC binding but not their effect on resistance; soluble factors caused a 50%-70% resistance decrease.

Conclusions:

  • Trypsin-resistant membrane components and soluble factors from Pf-IRBCs contribute to impaired BBB integrity in CM.
  • Pf-IRBCs impact BBB integrity through a multistep, multifactorial process.