Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Infectious entry pathway for canine parvovirus.

S Basak1, H Turner

  • 1Department of Microbiology, University of Alabama, Birmingham 35294.

Virology
|February 1, 1992
PubMed
Summary

Canine parvovirus (CPV) requires a low pH environment within endosomes for infection. This study shows that blocking endosomal acidification prevents CPV uncoating and viral replication.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Novel Roles for the Ectoenzyme CD38 in the Maintenance of Transcriptional and Metabolic Homeostasis in Astrocytes.

Glia·2025
Same author

Contact lens delivery improves therapeutic efficacy of nintedanib in rabbit eyes with alkali burn.

Experimental eye research·2025
Same author

Computational analysis of therapeutic potential for simplified Piper. spp- derived medicinal mixtures in anxiety, sleep, pain and seizure.

bioRxiv : the preprint server for biology·2025
Same author

A pathway to next-generation mast cell stabilizers identified through the novel Phytomedical Analytics for Research Optimization at Scale data platform.

bioRxiv : the preprint server for biology·2025
Same author

Hemp fibre reinforced natural rubber bio-macromolecule based biodegradable engineered leather.

International journal of biological macromolecules·2024
Same author

Biomarkers for personalised prevention of chronic diseases: a common protocol for three rapid scoping reviews.

Systematic reviews·2024

Area of Science:

  • Virology
  • Cell Biology
  • Infectious Diseases

Background:

  • Canine parvovirus (CPV) is a significant pathogen causing severe gastrointestinal disease in dogs.
  • The cellular entry mechanisms of many viruses involve endocytosis and subsequent fusion or pore formation, often dependent on endosomal pH.
  • Understanding CPV entry is crucial for developing targeted antiviral strategies.

Purpose of the Study:

  • To investigate the role of low pH-dependent pathways in canine parvovirus (CPV) entry into host cells.
  • To determine if endosomal acidification is necessary for CPV uncoating and initiation of infection.

Main Methods:

  • Utilized lysosomotropic bases (NH4Cl, chloroquine) to inhibit endosomal acidification.
  • Employed immunofluorescence, ultrastructural analysis, and biochemical assays to monitor CPV entry and replication.
  • Assessed viral DNA and antigen synthesis under different treatment conditions and time points.

Main Results:

  • CPV virions accumulated in large vacuoles in the presence of NH4Cl and chloroquine.
  • Ultrastructural studies revealed virion uptake via uncoated vesicles that fuse with larger compartments.
  • Inhibition of endosomal acidification prevented virion uncoating and significantly reduced viral DNA and antigen synthesis.
  • The inhibitory effect was most pronounced when cells were treated pre-infection, indicating a critical early step.

Conclusions:

  • Canine parvovirus (CPV) entry into host cells is dependent on exposure to a low pH environment within endosomes.
  • Acidification of endosomes is essential for CPV uncoating and the initiation of a productive infection.
  • These findings highlight a potential therapeutic target for CPV infections by interfering with endosomal acidification.

Related Experiment Videos