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Plasma biochemical reference intervals for koi.

Brian S Palmeiro1, Karen L Rosenthal, Gregory A Lewbart

  • 1Department of Clinical Studies-Philadelphia, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.

Journal of the American Veterinary Medical Association
|March 3, 2007
PubMed
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This study evaluated an in-house biochemical analyzer for adult koi, establishing reference intervals for plasma analytes. The analyzer showed good precision for most tests, aiding aquatic veterinary diagnostics.

Area of Science:

  • Aquatic animal health
  • Clinical biochemistry
  • Veterinary diagnostics

Background:

  • Accurate biochemical analysis is crucial for diagnosing diseases in koi.
  • Establishing reliable reference intervals for plasma analytes in adult koi is essential for health assessments.
  • In-house diagnostic tools can improve efficiency and accessibility in veterinary practice.

Purpose of the Study:

  • To evaluate the reproducibility of an in-house tabletop biochemical analyzer for measuring plasma biochemical analytes in adult koi.
  • To establish reference intervals for various plasma biochemical analytes in healthy adult koi.
  • To assess the clinical utility of the analyzer for aquatic veterinarians.

Main Methods:

  • A prospective study involving 71 healthy adult koi.

Related Experiment Videos

  • Plasma samples were analyzed for 19 biochemical analytes using an in-house tabletop analyzer.
  • Reproducibility was assessed using intraclass correlation coefficients and analysis of replicate sample differences.

    • Main Results:

    • The analyzer demonstrated excellent reproducibility for most analytes.
    • Good reproducibility was noted for alanine aminotransferase, while poor reproducibility was observed for sodium (Na), gamma-glutamyltransferase, and phosphorus (P).
    • Reference intervals were successfully established for several plasma biochemical analytes in adult koi.

    Conclusions:

    • The in-house biochemical analyzer exhibits good precision for most plasma analytes in adult koi.
    • Further validation is required for analytes with poor reproducibility (globulin, Na, P, K, albumin) before establishing definitive reference intervals.
    • The established reference intervals can serve as a valuable diagnostic tool for aquatic veterinarians in fish wellness programs.