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Updated: Jul 16, 2026
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
Published on: November 6, 2021
[Effect of verapamil on the proliferation of normal gingival fibroblast]
1Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China. luanqx@yahoo.com.cn
Objective:
To evaluate the effect of verapamil on the proliferation of normal gingival fibroblast (NGF) in vitro.
Methods:
NGF was isolated and cultured. The 5th passage of NGF was incubated with 0, 0.1, 1, 10, 100 micromol/L verapamil respectively. Methyl thiazolyl tetrazolium (MTT) assay and flow cytometry were used to detect cell proliferation and cell cycles.
Results:
Incubated with 100 micromol/L verapamil for 66 h, the A value of normal gingival fibroblast was significantly lower than those without verapamil groups (P < 0.01). Incubated with 100 micromol/L verapamil for 18 h, 69% of cells were at the G(0) - G(1) phase, 27% were at the S phase. For control group (without verapamil) 41% of cells were at G(0) - G(1) phase and 49% cells were at S phase. There was significant difference between the two groups (P < 0.001).
Conclusions:
100 micromol/L verapamil inhibited proliferation of normal gingival fibroblast by a cell-cycle arrest.
